Occurrence of T-2 toxin in processed cereals and pulses in Turkey determined by HPLC and TLC

OMURTAG G. Z., Yazıcıglu D.

Food Additives and Contaminants, vol.18, no.9, pp.844-849, 2001 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 9
  • Publication Date: 2001
  • Doi Number: 10.1080/02652030118172
  • Journal Name: Food Additives and Contaminants
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.844-849
  • Keywords: Cereals and pulses, HPLC, T-2 toxin, TLC
  • Istanbul Medipol University Affiliated: No


The purpose of this study was to investigate the T-2 toxin level of contaminated cereal and pulse products in Turkey. T-2 toxin was detected using high performance liquid chromatography (HPLC) with UV detection at 208 nm and thin layer chromatography (TLC) was used for confirmation of the T-2 toxin-contaminated samples (≥ ppm). The recovery was 93 ± 3.3% (SD 3.29, n = 5) for chickpea spiked with a known amount of T-2 toxin (1 ppm). The detection limits for T-2 toxin for HPLC and TLC were 25 ng per injection and 50 ng per spot, respectively. A total of 50 commercially available cereal and pulse product samples, collected from markets and street bazaars, were analysed. Incidences of T-2 toxin detected in cereal and pulse products were 23.5% and 31.2%, respectively and the maximum detected amount was 1.9 ppm in a sample of dried beans. The incidence of toxin-contaminated specimens is not so low relative to the volume of specimens produced. © 2001 Taylor & Francis Group, LLC.