Rapid detection of carbapenemases and eskapeec bacteria
Archives of the Balkan Medical Union, cilt.56, sa.2, ss.142-149, 2021 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 56 Sayı: 2
- Basım Tarihi: 2021
- Doi Numarası: 10.31688/abmu.2021.56.2.01
- Dergi Adı: Archives of the Balkan Medical Union
- Derginin Tarandığı İndeksler: Scopus, EMBASE, Directory of Open Access Journals
- Sayfa Sayıları: ss.142-149
- Anahtar Kelimeler: Antimicrobial drug resistance., Carbapenemase, Enterobacteriaceae, ESKAPEEc, Lactamase
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- İstanbul Medipol Üniversitesi Adresli: Evet
Özet
Introduction. Carbapenems have been the ultimate antibiotics for the treatment of infections caused by multidrug-resistant bacteria. However, recently, carbapenems-resistant bacteria have emerged significantly. The objective of the study was the identification of ESKAPEEc bacteria and rapid detection of carbapenemase production. Materials and methods. We tried a novel rapid test methodology that detects some carbohydrates metabolization associated with bacterial growth in the presence of imipenem. The formation of acid metabolites is evidenced by a color change of a pH indicator. Results. Carbapenemase production is phenotypically demonstrated in carbapenem-resistant bacterial strains. In the study, carbapenemase production was detected within 3 hours, and identification of ESKAPEEc bacteria was completed within 4 hours by carbohydrate metabolism. Conclusions. In conclusion, our cost-effective technique may provide a practical solution for the determination of multi-drug resistance by using the fermentation metabolism in bacteria.