Rapid detection of carbapenemases and eskapeec bacteria

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Aydogan O., Çakirlar F. K.

Archives of the Balkan Medical Union, vol.56, no.2, pp.142-149, 2021 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 56 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.31688/abmu.2021.56.2.01
  • Journal Name: Archives of the Balkan Medical Union
  • Journal Indexes: Scopus, EMBASE, Directory of Open Access Journals
  • Page Numbers: pp.142-149
  • Keywords: Antimicrobial drug resistance., Carbapenemase, Enterobacteriaceae, ESKAPEEc, Lactamase
  • Istanbul Medipol University Affiliated: Yes


Introduction. Carbapenems have been the ultimate antibiotics for the treatment of infections caused by multidrug-resistant bacteria. However, recently, carbapenems-resistant bacteria have emerged significantly. The objective of the study was the identification of ESKAPEEc bacteria and rapid detection of carbapenemase production. Materials and methods. We tried a novel rapid test methodology that detects some carbohydrates metabolization associated with bacterial growth in the presence of imipenem. The formation of acid metabolites is evidenced by a color change of a pH indicator. Results. Carbapenemase production is phenotypically demonstrated in carbapenem-resistant bacterial strains. In the study, carbapenemase production was detected within 3 hours, and identification of ESKAPEEc bacteria was completed within 4 hours by carbohydrate metabolism. Conclusions. In conclusion, our cost-effective technique may provide a practical solution for the determination of multi-drug resistance by using the fermentation metabolism in bacteria.