Effects of bioactive-rich extracts of pomegranate, persimmon, nettle, dill, kale and Sideritis and isolated bioactives on arachidonic acid induced markers of platelet activation and aggregation
Journal of the Science of Food and Agriculture, cilt.93, sa.14, ss.3581-3587, 2013 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 93 Sayı: 14
- Basım Tarihi: 2013
- Doi Numarası: 10.1002/jsfa.6328
- Dergi Adı: Journal of the Science of Food and Agriculture
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.3581-3587
- Anahtar Kelimeler: Flow cytometry, in vitro, Platelet activation, Platelet aggregation, Polyphenols
- İstanbul Medipol Üniversitesi Adresli: Hayır
Özet
BACKGROUND: The beneficial effect of fruit- and vegetable-rich diets on cardiovascular health is partly attributed to the effect of their bioactive compounds on platelet function. The aim of this study was to investigate the effects of bioactive-rich plant extracts and isolated bioactive metabolites on platelet function. Blood samples from healthy subjects (n=4) and subjects with metabolic syndrome (n=4) were treated with six extracts of bioactive-rich plants consumed as traditional foods in the Black Sea region, or with human metabolites of the bioactives quercetin and sulforaphane. Markers of arachidonic acid induced platelet activation and platelet-leucocyte aggregation were assessed using flow cytometry. RESULTS: In subjects with metabolic syndrome, kale extract significantly inhibited agonist induced P-selectin expression (P=0.004). Sulforaphane-cysteine-glycine, a human plasma metabolite of the related glucosinolate, glucoraphanin, significantly inhibited P-selectin and GPIIb-IIIa expression (P=0.020 and 0.024, respectively) and platelet-neutrophil aggregation (P=0.027). Additionally, pomegranate extract significantly inhibited GPIIb-IIIa expression (P=0.046) in subjects with metabolic syndrome. In healthy subjects only dill extract significantly inhibited agonist induced P-selectin expression (P=0.025). CONCLUSION: These data show that bioactive-rich extracts of kale and pomegranate that are consumed as traditional plant foods of Black Sea area countries were effective in modulating platelet function. © 2013 Society of Chemical Industry.