Effective management of both financial and operational performance is crucial for achieving high performance in health institutions. Effective performance management can lead to improved service delivery, patient recovery, and overall performance improvement. This study aims to assess the financial performance of a Ministry of Health hospital located in the Aegean region that was affiliated with a university hospital at the end of 2011. The study also aims to compare the financial performance data with the operational performance data of the hospital. The Ratio analysis method, which is widely accepted for measuring the financial performance of hospitals, is utilized in this study. Twelve ratio analysis methods used to measure financial performance were examined, and the financial performance of the hospital before and after the affiliation was compared and evaluated. Spearman correlation analysis was performed between the financial performance data and operational performance data of the hospital during the six-year operating period between 2009-2014. In conclusion, the healthcare institution faced challenges in meeting its short-term debt obligations and experienced cash shortages. The use of liabilities and debt was reduced after the affiliation, and the institution became profitable with successful management and health service delivery. Results from Spearman correlation analysis showed a positive correlation between the number of outpatients and return on equity and return on total assets. Similarly, the number of emergency admissions, surgeries, non-physician healthcare personnel, and childbirths were positively correlated with net profit margin, return on total assets, and return on equity. The number of radiological examinations and laboratory tests had significant correlations with the inventory turnover ratio.