PHYTOCHEMICAL AND IN VITRO PHARMACOLOGICAL EVALUATION OF PHLOMIS PUNGENS PHLOMIS PUNGENS’İN FİTOKİMYA VE İN VİTRO FARMAKOLOJİK ETKİLERİNİN DEĞERLENDİRİLMESİ


Creative Commons License

Okur M. E., KARADAĞ A. E., Reis R., Sipahi H., ARSLAN R., DEMİRCİ B., ...More

Ankara Universitesi Eczacilik Fakultesi Dergisi, vol.46, no.1, pp.11-22, 2022 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 46 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.33483/jfpau.975024
  • Journal Name: Ankara Universitesi Eczacilik Fakultesi Dergisi
  • Journal Indexes: Scopus, Central & Eastern European Academic Source (CEEAS), EMBASE, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.11-22
  • Keywords: Cell culture, Fibroblast, Inflammation, Lamiaceae, Macrophage
  • Istanbul Medipol University Affiliated: Yes

Abstract

Objective: This study aimed to investigate the in vitro wound healing, anti-inflammatory, antimicrobial and antioxidant activity of Phlomis pungens Willd. extract derived from the aerial parts. Material and Method: The phytochemical analysis was performed using GC-MS in order to identify the volatile components of the bioactive Hex extract. The wound healing activity of P. pungens extract was evaluated based on in vitro antimicrobial, antioxidant, anti-inflammatory, and, scratch activity was studied. In addition, the in vitro cytotoxicity of the extract was also evaluated. Result and Discussion: P. pungens methanol extract depicted a 5-LOX inhibitory activity at 78.2µg/mL (IC50), while the antioxidant activity by DPPH radical provided an IC50=2.41mg/mL, and the ABTS radical showed IC50=3.32mg/mL, respectively. The extract showed dose-dependently anti-inflammatory activity while L-NAME and P. pungens methanol extract significantly decreased LPS stimulated PGE2 production. According to the scratch assay results, all treatments led to an increase in cell migration rate with a dose-dependent effect. Our findings suggested that P. pungens methanol extract may have a role in wound healing according to the scratch test, and it is thought that its antioxidant and anti-inflammatory activity also contributed. Further evaluations are ongoing to confirm the in vitro activity under in vivo conditions.