GC-MS analysis and antimicrobial activity of alkaloid extract from Genista vuralii

Erdemoglu N., Ozkan S., Duran A., TOSUN F.

Pharmaceutical Biology, vol.47, no.1, pp.81-85, 2009 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 1
  • Publication Date: 2009
  • Doi Number: 10.1080/13880200802448674
  • Journal Name: Pharmaceutical Biology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.81-85
  • Keywords: Alkaloids, Antimicrobial activity, Fabaceae, GC-MS, Genista, Genista vuralii
  • Istanbul Medipol University Affiliated: No


In the present study, the alkaloid composition of the aerial parts of Genista vuralii A. Duran & H. Dural (Fabaceae) was investigated by capillary GC-MS. Ten quinolizidine alkaloids were identified by capillary GC-MS, namely, N-methylcytisine, cytisine, tetrahydrorhombifoline, 17-oxosparteine, 5,6-dehydrolupanine, lupanine, 17-oxolupanine, anagyrine, baptifoline, and 13α-tigloyloxylupanine. Among them, anagyrine (93.04) was the most abundant alkaloid. Furthermore, antibacterial and antifungal activities of the alkaloid extract of G. vuralii were tested against standard strains of bacteria (Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus) as well as fungi (Candida albicans, Candida krusei). The alkaloid extract of G. vuralii presented good activity against S. aureus, B. subtilis, and C. krusei, with minimum inhibitory concentrations (MIC) of 62.5 μg/mL. The remaining MIC values were found to range between 125 and 500 μg/mL. To the best of our knowledge, the current work is the first to report the alkaloid profile and antimicrobial activity of G. vuralii L. growing in Turkey. © 2009 Informa UK Ltd.