In vitro antitumor activity evaluation of some 1,2,4-triazine derivatives bearing piperazine amide moiety against breast cancer cells

YURTTAŞ L., Demirayak Ş., ILGIN S., Atli Ö.

Bioorganic and Medicinal Chemistry, vol.22, no.22, pp.6313-6323, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 22
  • Publication Date: 2014
  • Doi Number: 10.1016/j.bmc.2014.10.002
  • Journal Name: Bioorganic and Medicinal Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.6313-6323
  • Keywords: 1,2,4-Triazine, Piperazine amide, Breast cancer, Antiproliferative activity, Apoptosis, XTT method
  • Istanbul Medipol University Affiliated: Yes


A series of 1,2,4-triazine derivatives bearing piperazine amide moiety has been synthesized and investigated for their potential anticancer activities. 1-[4-(5,6-Bis(4-subtituted phenyl)-1,2,4-triazin-3-yl)piperazin-1-yl]-2-[4-(3-substituted phenyl)piperazin-1-yl]ethanone derivative (1-32) compounds were synthesized by a four step synthetic procedure. The activity studies were evaluated using XTT method, BrdU method and flow cytometric analysis on MCF-7 breast cancer cells and NIH/3T3 (mouse embryonic fibroblast cells) healthy cells. Compounds 5 with 3-chlorophenyl and compound 7 with 4-chlorophenyl substitutions were found to be promising antiproliferative agents comparing with an effective anticancer drug, cisplatin.