Evaluation of in situ gel containing pycnogenol for cutaneous wound healing Piknogenol ıçeren in situ jelin yara ıyileşme özelliği ıçin değerlendirilmesi

Okur M. E., Ayla Ş., BATUR Ş., Yoltaş A., Genç E., Pertek S., ...More

Medeniyet Medical Journal, vol.34, no.1, pp.67-75, 2019 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 1
  • Publication Date: 2019
  • Doi Number: 10.5222/mmj.2019.29053
  • Journal Name: Medeniyet Medical Journal
  • Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.67-75
  • Keywords: In situ gel, Mice, Pycnogenol, Wound healing
  • Istanbul Medipol University Affiliated: Yes


Aim: Pycnogenol® (PYC) are used for various medicinal purposes. The aims of the present study were to evaluate wound healing activity of PYC loaded in situ gel in mice and to investigate its antibacterial activity. Method: Temperature-sensitive in situ gel containing 5% PYC was formulated by cold method using Poloxamer 188, Poloxamer 407. Blank and drug loaded in situ gel formulations were evaluated for clarity, pH, viscosity, gelation temperature, gellation capacity. The wound healing effect was tested by in vivo wound model. PYC in situ gel was administrated topically at a concentration of 5% for the 10 consecutive days after skin injury. Wound closure was measured for 10 days and at 10th day wound healing was assessed by levels of angiogenesis, granulation tissue thickness, epidermal and dermal regeneration. Its antimicrobial property was evaluated by Agar well diffusion test. Results: The clarity, pH, viscosity, gellation capacity of in situ gels were found to be satisfactory.Results showed that PYC in situ gel exhibited remarkable wound healing activity with the 86.91% reduction of the wound area at the day 10 on the circular excision wound model compared to control group. Moreover PYC showed significant effect on angiogenesis, granulation tissue thickness, epidermal and dermal regeneration compared to the control group. In addition to this, PYC demonstrated antibacterial and antifungal activities. The most sensitive strains were B. cereus (23.66 mm), C. albicans (22.66 mm), and S. aueus (23 mm). Conclusion: Results indicated that PYC in situ gel enhanced wound healing effectively, and so it may be developed as a an effective agent to improve wound healing in future studies to be performed.