Therapeutic monitoring of isoniazid, pyrazinamide and rifampicin in tuberculosis patients using LC

Unsalan S., Sancar M., Bekce B., Clark P., Karagoz T., Izzettin F., ...More

Chromatographia, vol.61, no.11-12, pp.595-598, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 61 Issue: 11-12
  • Publication Date: 2005
  • Doi Number: 10.1365/s10337-005-0549-0
  • Journal Name: Chromatographia
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.595-598
  • Keywords: column liquid chromatography, therapeutic drug monitoring, isoniazid, tuberculosis treatment, pyrazinamide and rifampicin
  • Istanbul Medipol University Affiliated: No


The objective of this study was to measure plasma concentration of isoniazide (INH), pyrazinamide (PZA) and rifampisin (RIF) in tuberculosis patients by using HPLC. 100 μL of plasma was deproteinized by adding trichloroacetic acid and acetonitrile to yield INH, PZA and RIF respectively. They were analysed by HPLC using a reversed phase C18 pre-column linked to a 4 μm C18 analytical column with a gradient solvent programme, which delivered 3% to 40% (v/v) acetonitrile in phosphate buffer in 20 min at rate of 0.8 mL min-1. Signals were monitored by diode-array detector. Acetanilide was used as internal standard. The method is reproducible and accurate with lower limits of quantification of 0.6 mg L-1 for INH, 1.5 mg L-1 for PZA and 0.7 mg L-1 for RIF. The plasma of 25 patients receiving daily standard therapy were assayed for INH, PZA and RIF 3 h after administration. Plasma concentration were found between 0.98 and 6.27 mg L-1 for INH, 11.05 and 47.26 mg L-1 for PZA, 5.09 and 33.20 mg L-1 for RIF respectively. Many of the plasma levels were found to be sub therapeutic. This practical method may be used for monitoring drug plasma levels of patients who fail to respond to treatment. © 2005 Friedr. Vieweg & Sohn/GWV Fachverlage GmbH.