Determination of a novel integron-located variant (blaOXA-320) of Class D β-lactamase in Proteus mirabilis

ÇOPUR ÇİÇEK A., Duzgun A. O., Saral A., Sandalli C.

Journal of Basic Microbiology, vol.54, no.10, pp.1030-1035, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 54 Issue: 10
  • Publication Date: 2014
  • Doi Number: 10.1002/jobm.201300264
  • Journal Name: Journal of Basic Microbiology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1030-1035
  • Keywords: blaOXA-320 gene, Class D β-lactamase, Integron, P. mirabilis
  • Istanbul Medipol University Affiliated: Yes


Proteus mirabilis (P. mirabilis) is one of Gram-negative pathogens encountered in clinical specimens. A clinical isolate (TRP41) of P. mirabilis was isolated from a Turkish patient in Turkey. The isolate was identified using the API 32GN system and 16S rRNA gene sequencing and it was found resistant to ampicillin/sulbactam, piperacillin, tetracycline, and trimethoprim/sulfamethoxazole. This isolate was harboring a Class 1 integron gene cassette and its DNA sequence analysis revealed a novel blaOXA variant exhibiting one amino acid substitution (Asn266Ile) from blaOXA-1. This new variant of OXA was located on Class 1 integron together with aadA1 gene encoding aminoglycoside-modifying enzymes. According to sequence records, the new variant was named as blaOXA-320. Cassette array and size of integron were found as blaOXA-320-aadA1 and 2086bp, respectively. The blaOXA-320 gene is not transferable according to conjugation experiment. In this study, we report the first identification of blaOXA-320-aadA1 gene cassette, a novel variant of Class D β-lactamase, in P. mirabilis from Turkey.