Practical approach for detection and identification of OXA-10-derived ceftazidime-hydrolyzing extended-spectrum β-lactamases

VAHABOĞLU H., ÖZTÜRK R., Akbal H., Saribas S., Tansel O., Coskunkan F.

Journal of Clinical Microbiology, vol.36, no.3, pp.827-829, 1998 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 3
  • Publication Date: 1998
  • Doi Number: 10.1128/jcm.36.3.827-829.1998
  • Journal Name: Journal of Clinical Microbiology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.827-829
  • Istanbul Medipol University Affiliated: Yes

Abstract

A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 β-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of bla(OXA) was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.