The aims of this study were to compare film and digital radiography in assessing the radiopacities of root canal sealers and to establish the relation in aluminum equivalent values of different methods. Standard disks of 5 different sealers were exposed together with an aluminum step wedge by using occlusal films and storage phosphor plates. Optical density of the sealers was evaluated by transmission densitometry, and mean gray values were determined by digital analysis. The data were analyzed by using two-way analysis of variance (P = .05). Pairwise comparisons were made by using Tukey post hoc and paired t tests (P = .05). The order from the most radiopaque to the most radiolucent sealer was the same for both methods; however, aluminum equivalent values determined by transmission densitometry were significantly higher (P < .01). Aluminum equivalent values of the 2 radiographic methods were 7%-20% different. The International Standards Organization standard for the radiopacity of dental root canal sealing materials needs modifications for digital systems. © 2008 American Association of Endodontists.