Micro-spoon technique in estimation of sperm concentration in rat epididymis
Tohoku Journal of Experimental Medicine, cilt.191, sa.1, ss.1-5, 2000 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 191 Sayı: 1
- Basım Tarihi: 2000
- Doi Numarası: 10.1620/tjem.191.1
- Dergi Adı: Tohoku Journal of Experimental Medicine
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1-5
- Anahtar Kelimeler: Cauda epididymis, Micro-spoon, Sperm concentration
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- İstanbul Medipol Üniversitesi Adresli: Hayır
Özet
Several technical procedures were described for estimation of epididymal sperm concentration until today. In all of the described techniques, epididymal material was drawn into a micro-pipette before counting procedure. We aimed to proceed this stage without using any pipette because of the difficulty in aspirating very viscous epididymal material into a micro-pipette. For measuring epididymal material we used a 2 μl reservoir which we named as a micro-spoon. It was filled with the epididymal material and layered in a tube with Ringer's solution of 2 ml containing sodium hyaluronate. Then, it was mixed in order to obtain 1/1000 diluted suspension. Sperm cells in this suspension were counted on a Makler chamber under a light microscope. The procedure was easy and time saving, compared to the other techniques. © 2000 Tohoku University Medical Press.