Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing β-lactamases in Pseudomonas aeruginosa in Turkey

Iraz M., Duzgun A. O., ÇOPUR ÇİÇEK A., Bonnin R. A., Ceylan A., Saral A., ...More

Diagnostic Microbiology and Infectious Disease, vol.78, no.3, pp.292-294, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 78 Issue: 3
  • Publication Date: 2014
  • Doi Number: 10.1016/j.diagmicrobio.2013.12.003
  • Journal Name: Diagnostic Microbiology and Infectious Disease
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.292-294
  • Keywords: β-lactamase, Carbapenemase, Class 1 integron, ESBL, Pseudomonas aeruginosa
  • Istanbul Medipol University Affiliated: Yes


Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = 1). PCR and DNA sequence analysis revealed that 1 strain possessed the blaGES-5 and another carried a novel blaVIM variant, named VIM-38. This new gene exhibited 1 amino acid substitution (Ala265Val) in comparison to its closest variant, VIM-5. Both VIM encoding genes were clones and demonstrated similar susceptibility profile when expressed in identical background. The presence of VIM-38 increases the diversity of carbapenemases in Turkey. © 2014 Elsevier Inc.