Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing β-lactamases in Pseudomonas aeruginosa in Turkey

Iraz M., Duzgun A. O., ÇOPUR ÇİÇEK A., Bonnin R. A., Ceylan A., Saral A., ...Daha Fazla

Diagnostic Microbiology and Infectious Disease, cilt.78, sa.3, ss.292-294, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 78 Sayı: 3
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.diagmicrobio.2013.12.003
  • Dergi Adı: Diagnostic Microbiology and Infectious Disease
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.292-294
  • Anahtar Kelimeler: β-lactamase, Carbapenemase, Class 1 integron, ESBL, Pseudomonas aeruginosa
  • İstanbul Medipol Üniversitesi Adresli: Evet

Özet

Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = 1). PCR and DNA sequence analysis revealed that 1 strain possessed the blaGES-5 and another carried a novel blaVIM variant, named VIM-38. This new gene exhibited 1 amino acid substitution (Ala265Val) in comparison to its closest variant, VIM-5. Both VIM encoding genes were clones and demonstrated similar susceptibility profile when expressed in identical background. The presence of VIM-38 increases the diversity of carbapenemases in Turkey. © 2014 Elsevier Inc.