Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing β-lactamases in Pseudomonas aeruginosa in Turkey
Diagnostic Microbiology and Infectious Disease, cilt.78, sa.3, ss.292-294, 2014 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 78 Sayı: 3
- Basım Tarihi: 2014
- Doi Numarası: 10.1016/j.diagmicrobio.2013.12.003
- Dergi Adı: Diagnostic Microbiology and Infectious Disease
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.292-294
- Anahtar Kelimeler: β-lactamase, Carbapenemase, Class 1 integron, ESBL, Pseudomonas aeruginosa
- İstanbul Medipol Üniversitesi Adresli: Evet
Özet
Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = 1). PCR and DNA sequence analysis revealed that 1 strain possessed the blaGES-5 and another carried a novel blaVIM variant, named VIM-38. This new gene exhibited 1 amino acid substitution (Ala265Val) in comparison to its closest variant, VIM-5. Both VIM encoding genes were clones and demonstrated similar susceptibility profile when expressed in identical background. The presence of VIM-38 increases the diversity of carbapenemases in Turkey. © 2014 Elsevier Inc.