Akademik Veri Yönetim Sistemi
Istanbul Journal of Pharmacy, cilt.50, sa.2, ss.103-110, 2020 (ESCI)
Background and Aims: Styphnolobium japonicum (L.) Schott. (Sophora japonica) is a medicinal plant applied for variousdiseases, in the traditional medicine field. The evaluation of methanol extract of S. japonicum root derived from the PharmaGrade plant drug, was performed in terms of various in vitro biological activities.Methods: The LC-MS analysis was used for the chemical characterization of the methanol extract. The anti-cancer activitywas evaluated in colon (HT-9), brain (U-87), and prostate (PC-3) cancer cells by Cell Titer Glo viability assay (Promega) andwestern blot analysis of PARP (Poly ADP-ribose polymerase) cleavage.Results: The relative amounts of matrine and oxymatrine in the extract were found as 0.49±0.006 mg/mL and 0.27±0.016 mg/mL, respectively. The S. japonicum extract showed 53.17±0.97 mg of gallic acid (GA)/g corresponding to the total phenolicamounts, resulting in relatively moderate antioxidant activity (1.94±0.23 and 2.79±0.15 mg/mL) on the in vitro2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS•) and 2,2-diphenyl-1-picrylhydrazyl (DPPH•) assays. Treatment with 10 mg/mLS. japonica root extract for 24h resulted in a significant decrease in cell viability. The cell viability of U-87, HT-29, and PC-3cancer cell lines was determined as 35±2.21%, 14±2.11%, and 46±5.67%, respectively. The extract showed 5.104, 5.012 and0.555 mg/mL IC50 values for HT-29, U-87, and PC-3 cell lines, respectively. Particularly, the IC50 value of PC-3 cancer cell linewas significantly lower than the healthy human fibroblast cells. In further, the apoptosis in S. japonicum root extract treatedPC-3 cells was detected through flow cytometry analysis of Annexin V positive cells and western blot analysis of PARP cleavage.Conclusion: It can be concluded that the methanol extract in determined doses induces the apoptosis of the PC-3 cancer cells,without any significant cytotoxic effect on healthy human fibroblast cells. In addition, the LCMS analysis showed the presenceof matrine and oxymatrine, which are known for their anticancer activity. To the best of our knowledge, these are the firstpreliminary results indicating the possible use of S. japonicum root extract. Thus, the methanol extract can be further studiedfor its therapeutic potential of primarily prostate and other cancer types.