Objectives: Aronia Melanocarpa is a polyphenolic herbal ingredient with anti-inflammatory,

anticancer, anti-microbial, anti-viral, anti-diabetic and anti-platelet properties. While the hypothesis of this study is that Aronia Melanocarpa will reduce the amount of periodontopathogens, the aim of the study is to evaluate the antibacterial effect of aronia on certain pathogens in vitro. Materials and Methods:Periodontopathogen species which are; Prevotella intermedia, Fusobacterium nucleatum and Porphyromonas gingivalis and also Streptococcus mutans obtained from clinical specimens were included in the study. Before the experiments, the strain needed to be activated. Aronia extract (100% pure), 1/10 extract (diluted in pure water) and 1/1000 extract (diluted in pure water) were tested at different concentrations, while sterile distilled water for the negative control group and paradontax mouth rinse for the positive control group was used. Agar well diffusion method was used for the determination of antimicrobial susceptibility of test groups. In the statistical analysis results, the significance was evaluated at the p<0.05 level. Results: As a result of the agar well diffusion technique, it was observed that the Aronia extract (%100 pure) possessed high antibacterial activity effectively against P. intermedia and F. nucleatum when compared with the 1/10 and 1/1000 extract groups (p<0.05). It was determined that all experimental groups did not have a statistically significant effect on P. gingivalis and S. Mutans (p>0.05). Conclusions: The findings of this study suggest that Aronia Melanocarpa can be used as an alternative to rinsing mouth wash with proven positive effects on gingival health and antimicrobial effect. At the same time, the results of this study can provide a reference for the further development of plant-derived antibacterial agents.