Detection of chromosomal abnormalities on interface cells from myeloid leukemias by FISH

ÖZDİLLİ K., Aydin F., Beşişik S. K., Oǧuz F., Temurhan S., Çako Ö., ...More

Nobel Medicus, vol.6, no.3, pp.84-89, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 3
  • Publication Date: 2010
  • Journal Name: Nobel Medicus
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.84-89
  • Keywords: FISH, chromosomal aberration, myeloid leukaemia
  • Istanbul Medipol University Affiliated: No


• Objective: Chronic myeloid leukemia (CML) was the first cancel associated with specific cytogenetic abnormalities, and a variety of chromosomal abnormalities in acute myeloid leukemia (AML), such as numerical and structural alterations and balanced translocations, have also been described. • Material and Method: In this study, blood or done marrow samples of 99 patients diagnosed with myeloid leukemia between 2000-2006 (CML n=57, AML n=42) were evaluated retrospectively using fluorescent in situ hybridization (FISH). All AML and CML patients included in the study group were analyzed for trisomy 8, Philadelphia (Ph) chromosome were analyzed in 57 CML patients, and 34 of the AML patients were analyzed for -7/del7q and - 5/del5q abnormalities. • Results: Of the 99 patients diagnosed with CML and AML, 53.55, were male and 46.5% were female with an average age of 40±17 years. The presence of +8 was detected in 29.8% of the CML patients, and 70.2% tested positive for Ph chromosome. The Ph+ chromosome ratio was 82.1% in male patients and 58.6% in female patients (p<0.05; OR=0.3). The +8 abnormality was detected in 46.34% of AML patients, but there was no significant difference in the +8 frequency between males and females (p=0.8). The -7 abnormality was the only abnormality detected in three AML patients; +8, del7q and -5 were detected in one patient; +8 and del7q were detected in two patients; and -5 alone was detected in one patient. • Conclusion: In conclusion, the high frequency of +8 in CML and AML cases highlights the necessity for routine, analysis of these abnormalities.