Determination and validation of ketoprofen, pantoprazole and valsartan together in human plasma by high performance liquid chromatography

Kocyigit-Kaymakcoglu B., Ünsalan S., Rollas S.

Pharmazie, vol.61, no.7, pp.586-589, 2006 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 61 Issue: 7
  • Publication Date: 2006
  • Journal Name: Pharmazie
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.586-589
  • Istanbul Medipol University Affiliated: No


A rapid and specific high-performance liquid chromatographic method was developed and validated for the simultaneous determination of ketoprofen, valsartan and pantoprazole in human plasma. Chromatographic separation of ketoprofen, valsartan and pantoprazole was performed using a Chromasil C 18 column (250 mm × 4.6 mm i.d., 5 μm particle size). The mobile phase consisted of a mixture of 0.02 M sodium dihydrogen phosphate buffer (pH 3.15) and acetonitrile (58:42, v/v) pumped through the chromatographic system at a flow rate of 1 mL · min-1. The Diode Array detector was operated at 225 and 272 nm. Rofecoxib was used as an internal standard. Sample treatment procedure consisted of deproteinisation with acetonitrile-methanol (50:50 v/v). Analytical recoveries were in the range of 79.00-118.00% of nominal values of valsartan, ketoprofen and pantoprazole. The method was reproducible and accurate with lower limits of quantification 250 μg · L-1 for pantoprazole and 500 μg · L -1 for ketoprofen and valsartan. This method was relatively easy to perform and allows simultaneous determination of these three drugs in plasma at nanogram levels.