Six miRNA expressions in the saliva of smokers and non-smokers with periodontal disease

Öngöz Dede F., Gökmenoğlu C., TÜRKMEN E., BOZKURT DOĞAN Ş., Ayhan B. S., Yildirim K.

Journal of Periodontal Research, vol.58, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 58
  • Publication Date: 2022
  • Doi Number: 10.1111/jre.13081
  • Journal Name: Journal of Periodontal Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, CINAHL, EMBASE, MEDLINE, Veterinary Science Database
  • Keywords: gingivitis, miRNA, periodontal therapy, periodontitis, smoking
  • Istanbul Medipol University Affiliated: Yes


Background: It has been stated that microRNA (miRNA) plays an important role in development, homeostasis, and immune functions, and abnormal miRNA expression may cause faster disease progression. Objective: The aim of this study was to determine miR-203, miR-142-3p, miR-146a, miR-146b, miR-155, and miR-29b gene expressions in the saliva of smokers and non-smokers with the periodontal disease before and after non-surgical periodontal therapy (NSPT). Methods: A total of 90 individuals, 30 with periodontitis, 30 with gingivitis, and 30 periodontally healthy (control group), were included. These three groups were divided into subgroups as smoking and non-smoking individuals, with 15 people in each group. NSPT was applied to patients with periodontitis and gingivitis. Saliva samples and clinical parameters were obtained at baseline and repeated 6 weeks after NSPT. Results: Saliva miR-203, miR-142-3p, miR-146a, miR-146b, and miR-155 gene expressions were significantly upregulated in patients with periodontal disease compared to the control group both in smokers and non-smokers, and also these miRNAs' gene expressions were significantly higher in the periodontitis group than in the gingivitis group at baseline (p <.05). A significant increase in saliva miR-142-3p expression was detected in all groups of smokers compared to non-smokers (p <.05). Although there was a decrease in salivary miRNAs gene expressions with the treatment, it was not statistically significant (p >.05). Conclusions: These results suggest that salivary miR-146a, miR-146b, miR142-3p, miR-155, and miR-203 gene expressions increased with the progression of periodontal disease, but unchanged after periodontal treatment. Moreover, smoking may contribute to an increase in the levels of salivary miR-142-3p in the periodontal health and disease.