Dose-Dependent Antiproliferation and αvβ3 Expression Downregulation by Phenylboronic Acid in SK-OV-3 and MIA-Pa-Ca-2

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Kolbaşı B., Acar T. Y., BÜLBÜL M. V., Polat A., Sapmaz K., keskin i.

Clinical and Experimental Health Sciences, cilt.16, sa.1, ss.1-9, 2026 (ESCI, TRDizin)

Özet

Objective: Metastasis, a critical process in cancer progression, is mediated predominantly by integrins, including the αvβ3 integrin, which plays a key role in cellular adhesion, migration, and survival. Both cell lines have significant metastatic potential, and αvβ3 integrin plays a role in their interaction with the extracellular matrix and invasiveness. This study investigated the antiproliferative potential of phenylboronic acid in SK-OV-3 ovarian adenocarcinoma and MIA-Pa-Ca-2 pancreatic carcinoma cell lines and its effects on integrin αvβ3 expression. Methods: SK-OV-3 and MIA-Pa-Ca-2 cells were cultured under standard conditions. Cells were treated with phenylboronic acid concentrations of 10–500 μM for cell viability analysis (MTT) and 50, 100, 500 μM for immunofluorescence staining analyses. An immunofluorescence assay was performed to assess αvβ3 integrin expression levels, and fluorescence intensity was analyzed as a surrogate marker of protein expression. Results: MTT analysis showed that the IC₅₀ values of PBA after 24 h exposure were 264.8 μM for SK-OV-3 and 294.4 μM for MIA-Pa-Ca-2 cells. Immunofluorescence revealed a dose-dependent decrease in αvβ3 integrin intensity in both cell lines (p<.0001). In SK-OV-3 cells, 50 μM PBA reduced fluorescence to ~45% of control, whereas MIA-Pa-Ca-2 required 100–500 μM for similar effects. Conclusion: This study demonstrated that phenylboronic acid exerts a dose-dependent antiproliferative effect in SK-OV-3 and MIA-Pa-Ca-2 cells and is associated with a concentration-dependent reduction in αvβ3 immunofluorescence signal, particularly at lower doses in SK-OV-3 cells. Given the well-described role of αvβ3 in cell adhesion, migration and metastatic spread, these observations raise the possibility that PBA may influence metastasis-related pathways, a hypothesis that should be tested in future functional studies.