In Vivo Molecular Bioluminescence Imaging: New Tools and Applications

Mezzanotte L., van ‘t Root M., KARATAŞ BRISTOW H., Goun E. A., Löwik C. W.

Trends in Biotechnology, vol.35, no.7, pp.640-652, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Review
  • Volume: 35 Issue: 7
  • Publication Date: 2017
  • Doi Number: 10.1016/j.tibtech.2017.03.012
  • Journal Name: Trends in Biotechnology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.640-652
  • Keywords: bioluminescence imaging, caged-luciferin, in vivo imaging, light producing transgenic animals, luciferase
  • Istanbul Medipol University Affiliated: Yes

Abstract

in vivo bioluminescence imaging (BLi) is an optical molecular imaging technique used to visualize molecular and cellular processes in health and diseases and to follow the fate of cells with high sensitivity using luciferase-based gene reporters. The high sensitivity of this technique arises from efficient photon production, followed by the reaction between luciferase enzymes and luciferin substrates. Novel discoveries and developments of luciferase reporters, substrates, and gene-editing techniques, and emerging fields of applications, promise a new era of deeper and more sensitive molecular imaging.