Are biological agents toxic to human chondrocytes and osteocytes?


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Isyar M., Bilir B., Yilmaz I., Cakmak S., Sirin D. Y., Guzelant A. Y., ...More

Journal of Orthopaedic Surgery and Research, vol.10, no.1, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 10 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.1186/s13018-015-0264-y
  • Journal Name: Journal of Orthopaedic Surgery and Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Biological agents, Chondrotoxicity, Osteotoxicity, Primary cell culture
  • Istanbul Medipol University Affiliated: Yes

Abstract

Purpose: The aim of the present study is to investigate the effects of biological agents (BAs) on human chondrocytes and osteocytes in vitro. Methods: Primary cell cultures obtained from gonarthrosis patients were divided into four groups, two of which were designated as control cultures of chondrocyte and osteocyte, and the other two groups were exposed to BAs administered via the culture medium. Cultured cells were characterized by immunophenotyping. Before and after administration of the agents, the cultures were observed by inverted and environmental scanning electron microscopy (ESEM). The number of live cells and the proliferation rate were monitored by MTT assay. Results: Rituximab and adalimumab were the least toxic agents to chondrocytes, whereas adalimumab and etanercept were to osteocytes. Conclusion: During periods of intense active inflammation, the concentration of the preferred BAs after inhibition of inflammation needs to be emphasized when their effects on cartilage and bone tissue are considered at the cellular level if the clinical practice is to continue.