Effects of Water Samples in Polyethylene Terephthalate Bottles Stored at Different Conditions on Zebrafish Embryos with Relevance to Endocrine Disrupting Chemical Migration and Adenomatous Polyposis Coli Tumor Suppressor Gene


ÜSTÜNDAĞ Ü. V., ÜNAL İ., ATEŞ P. S., TIRPANCI A., EĞİLMEZER G., ALTURFAN A. A., ...More

Clinical and Experimental Health Sciences, vol.9, no.2, pp.171-177, 2019 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 2
  • Publication Date: 2019
  • Journal Name: Clinical and Experimental Health Sciences
  • Journal Indexes: TR DİZİN (ULAKBİM)
  • Page Numbers: pp.171-177
  • Keywords: Polyethylene terephthalate, phthalates, Wnt/beta-catenin signaling, adenomatous polyposis coli
  • Istanbul Medipol University Affiliated: Yes

Abstract

Objective: Polyethylene terephthalate (PET) is a material that is most commonly used for production of clear plastic bottles. Adenomatous polyposiscoli (APC) and β-catenin have been related with cancer. Aim was to investigate the effects of PET bottled water samples that were exposed to sunlightand hot water on zebrafish embryos. Moreover the effects of these water samples on APC knockdown zebrafish embryos were also evaluated.Methods: Phthalate concentrations in water samples were determined using ELISA. Immunohistochemical method and RT-PCR were used toanalyse the expressions of proliferative cell nuclear antigen (PCNA), β catenin, Wnt 3a and Gsk3β. Biochemical parameters were measured usingspectrophotometric methods. Vitellogenin concentrations were measured using ELISA and apoptotic cells were evaluated by Acridine Orangestaining.Results: Increased PCNA, β-catenin, Wnt 3a, Gsk3β expressions, vitellogenin, nitric oxide, apoptosis and impaired oxidant-antioxidant balance wereobserved in the exposure groups with these increases being more profound in APC knockdown groups.Conclusion: APC knockdown embryos were more prone to the deleterious effects of water samples used in this study.