Evaluation of DNA damage in construction-site workers occupationally exposed to welding fumes and solvent-based paints in Turkey
Toxicology and Industrial Health, cilt.26, sa.9, ss.601-608, 2010 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 26 Sayı: 9
- Basım Tarihi: 2010
- Doi Numarası: 10.1177/0748233710374463
- Dergi Adı: Toxicology and Industrial Health
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.601-608
- Anahtar Kelimeler: DNA damage, construction site workers, welding fumes, solvent-based paints, comet assay
- İstanbul Medipol Üniversitesi Adresli: Hayır
Özet
In this study, the comet assay was used to evaluate whether welding fume and solvent base paint exposure led to DNA damage in construction-site workers in Turkey. The workers (n = 52) were selected according to their exposure in the construction site and controls (n = 26) from the general population, with no history of occupational exposure. The alkaline comet assay, a standard method for assessing genotoxicity, has been applied in peripheral lymphocytes of all subjects. The mean percentages of DNA in tail (%DNAT) of each group were evaluated, including the comparisons between smokers in each different group and the duration of exposure. Significant increase in the mean %DNAT(p < 0.01) was observed in all exposed subjects (12.34 ± 2.05) when compared with controls (6.64 ± 1.43). Also %DNATwas significantly high (p < 0.01) in welders (13.59 ± 1.89) compared with painters (11.10 ± 1.35). There was a statistical meaningful difference in % DNATbetween control and exposed smokers. Our findings indicate that exposure to welding fumes and paints induce genotoxic effect in peripheral lymphocytes, indicating a potential health risk for workers. Therefore, to ensure maximum occupational safety, biomonitoring is of great value for assessing the risk for construction workers. © 2010, SAGE Publications. All rights reserved.