Curcumin effects on cell proliferation, angiogenesis and metastasis in colorectal cancer

Calibasi-Kocal G., Pakdemirli A., BAYRAK S., Ozupek N. M., Sever T., BAŞBINAR Y., ...More

Journal of B.U.ON., vol.24, no.4, pp.1482-1487, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 24 Issue: 4
  • Publication Date: 2019
  • Journal Name: Journal of B.U.ON.
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1482-1487
  • Keywords: colorectal cancer, curcumin, invasion, migration, proliferation
  • Istanbul Medipol University Affiliated: Yes

Abstract

Purpose: Curcumin is a natural phytopolyphenol compound isolated from the root of turmeric (Curcuma longa) and possesses a wide range of biological properties. The purpose of this study was to evaluate the antiproliferative, wound healing, anti-invasive and anti-migrative effects of curcumin on HCT-116 and LoVo colorectal cancer cell lines. Methods: The antiproliferative activity of 2.5-75 µM curcumin was tested on HCT-116 and LoVo colorectal cell lines and the viability of the cells was tested with WST-1 reagent by using ELISA plate reader at 450 nm. xCELLigence RTCA DP system was used for the detection of anti-invasive and anti-migrative effects of curcumin. Results: The IC50 of curcumin was 10±0.03 for HCT-116 and 20±0.05 µM for LoVo cell lines. The IC50 of curcumin (10µM for HCT-116 and 20 µM for LoVo) showed anti-metastatic activity on these cell lines. Conclusion: This study showed that curcumin could be evaluated as a promising anti-cancer agent for human colorectal cancer.