Assesment of castellani solution's ototoxic effects on Guinea Pigs using otoacoustic emission and auditory evoked brainstem potentials

Bayir O., KIRKIM G., Mungan S., Gurkan S., KOLATAN H. E., ŞERBETÇİOĞLU M. B.

Journal of International Advanced Otology, vol.8, no.3, pp.345-353, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 3
  • Publication Date: 2012
  • Journal Name: Journal of International Advanced Otology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.345-353
  • Istanbul Medipol University Affiliated: No


Objective: We planned to evaluate the ototoxic effects of Castellani's solution on guinea pigs when used intratympanically. Materials and Methods: Fourteen female adult albino guinea pigs were used in this experiment. All treatments were performed in seven consecutive days using intratympanic injection. The first group comprised of left ears of seven guinea pigs and received 0. 2 ml of gentamicin. The second group comprised the left ears of the other seven guinea pigs and received 0. 1 ml of Castellani's solution. Contralateral ears of all these fourteen guinea pigs were used as negative control group and received 0. 2 ml of saline solution intratympanically. DPOAEs and ABRs were recorded three times for each guinea pig, prior to intratympanic injection, on the tenth and twenty-first days after the injections. Results: In Groups 1 and 2, DPOAE were absent on tenth and twenty-first days of the experiment. However, for Group 3, there was no significant change in both responses for the same time periods before and after the injections. On the tenth and twenty-first day of the experiment, in Groups 1 and 2, ABRs thresholds were found to be significantly increased. According to statistical analysis, significant differences were found between Groups 1 and 3 as well as between Groups 2 and 3. Conclusion: It is concluded that Castellani's solution has ototoxic effect on guinea pigs. Thus, based on this conclusion, Castellani's solution can not be considered to be safe to use patients with tympanic membrane perforation. © The Mediterranean Society of Otology and Audiology.