Comparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl

Sibai M., Altuntaş E., Süzek B. E., Şahin B., Parlayan C., ÖZTÜRK G., ...More

Biochemical and Biophysical Research Communications, vol.522, no.2, pp.428-434, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 522 Issue: 2
  • Publication Date: 2020
  • Doi Number: 10.1016/j.bbrc.2019.11.118
  • Journal Name: Biochemical and Biophysical Research Communications
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.428-434
  • Keywords: Proteomics, Axolotl, Limb regeneration, Metamorphosis, Gene expression profile
  • Istanbul Medipol University Affiliated: Yes


The axolotl (Ambystoma mexicanum) salamander, a urodele amphibian, has an exceptional regenerative capacity to fully restore an amputated limb throughout the life-long lasting neoteny. By contrast, when axolotls are experimentally induced to metamorphosis, attenuation of the limb's regenerative competence is noticeable. Here, we sought to discern the proteomic profiles of the early stages of blastema formation of neotenic and metamorphic axolotls after limb amputation by employing LC-MS/MS technology. We quantified a total of 714 proteins and qRT-PCR for selected genes was performed to validate the proteomics results and provide evidence for the putative link between immune system activity and regenerative potential. This study provides new insights for examination of common and distinct molecular mechanisms in regeneration-permissive neotenic and regeneration-deficient metamorphic stages at the proteome level.