Microplastics(MP), which can be of various types, sizes or shapes, are defined as synthetic polymer particles or fibers. There are studies on the amount and types of MP contained in many materials and toothpastes used in the field of dentistry. The aim of this study is to investigate the types and amounts of MP from the bristles that may occur during brushing, which is thought to cause gingival diseases by

penetrating the gingival sulcus. Toothbrushes (TEPE Select, Signal Performance,Banat Deep Clean, Oral-B Pro Expert,Colgate Extra Clean and Sensodyne GumCare Medium) selected with the brushing simulation applied on the monolithic zirconium dental arch model obtained in 3D were applied twice a day for 2minutes for 7days. Brush bristles were analyzed under Fouirer Transform Infrared Spectrophotometer (FTIR)(IRTracer-100) to determine which plastic material these bristles were made of. The samples collected from the bristles washed with deionized water were filtered and the MP amounts and types on the filters were analyzed under a Stereo Microscope(ZEISS 508). In the analysis of brush bristles with FTIR, Tepe was found to be 93%, Signal 88.6%, Colgate 88% nylon; Banat 91.6%, Sensodyne 92.1%, Oral-B 92.1% polybutylene terephthalate (PBT),. Statistically significant differences were observed between total MP, fragment, pellet and film results of different brushes (respectively: p<0.0001,p<0.0001,p<0.0001,p<0.0001;p<0.05). When the total MP release of different brushes on the first day is evaluated, It was observed that Oral-B released statistically significantly less MP than Colgate,Banat,Signal, and Tepe (respectively: p<0.0001,p<0.0001,p<0.0001,p:0.0069;p<0.05). In the study conducted with different brush brands, it was determined that there was MP release from the brush bristles in different sizes and shapes from the first day. When the amount of MP released on the first day and the amount of MP released on the 7th day were compared, a statistically significant increase was found in brushing with Sensodyne.