In vitro anti-inflammatory activities of Tanacetum parthenium L. extract and its major metabolite parthenolide


BAYDAR R., KARADAĞ A. E., BİLTEKİN KALELİ S. N., Güzelmeriç E., DEMİRCİ F.

Acta Pharmaceutica Sciencia, vol.62, no.1, pp.183-192, 2024 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 62 Issue: 1
  • Publication Date: 2024
  • Doi Number: 10.23893/1307-2080.aps6212
  • Journal Name: Acta Pharmaceutica Sciencia
  • Journal Indexes: Scopus, EMBASE
  • Page Numbers: pp.183-192
  • Keywords: anti-inflammatory, COX, feverfew, LOX, Tanacetum parthenium
  • Istanbul Medipol University Affiliated: Yes

Abstract

Tanacetum parthenium L. (Feverfew) is daisy-like Asteraceae plant carrying sesquiterpene lactones; used for the treatment of migraine and anti-inflamma-tory effect. This study aims to evaluate T. parthenium extract and major metabolite parthenolide for in vitro COX-1/COX-2, LOX inhibitory activity. The extract analyzed by HPTLC. To evaluate COX-1/COX-2 inhibition assays, stud-ied with commercial kits (20µg/mL concentration for extract, 5 µg/mL for par-thenolide). The major component of extract characterized as parthenolide. IC50 values for COX-1/COX-2 inhibition of extract were 10.45 and 9.81µg/mL, for parthenolide; 4.86 and 1.90µg/mL. SI values of T. parthenium extract and par-thenolide were 0.93 and 0.39. Extract showed selective COX-2 inhibitory activ-ity. The inhibition value of extract on LOX was 80% and inhibition value of par-thenolide was 41.13%. The results suggested that T. parthenium extract showed selective potential for COX-2 enzyme inhibition. To the best of our knowledge, the extract was tested with COX-1, COX-2, and LOX enzymes for the first time.