Development of a Chemogenetic Approach to Manipulate Intracellular pH

Ghaffari Zaki A., Miri S. M., Çimen Ş., Akgül Çağlar T., YİĞİT E. N., AYDIN M. Ş., ...Daha Fazla

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, cilt.145, sa.22, ss.11899-11902, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 145 Sayı: 22
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1021/jacs.3c00703
  • Dergi Adı: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Compendex, Computer & Applied Sciences, DIALNET
  • Sayfa Sayıları: ss.11899-11902
  • İstanbul Medipol Üniversitesi Adresli: Evet

Özet

Chemogenetic Operation of iNTRacellular prOton Levels (pH-Control) is a novel substrate-based enzymatic method that enables precise spatiotemporal control of ultralocal acidification in cultured cell lines and primary neurons. The genetically encoded biosensor SypHer3s showed that pH-Control effectively acidifies cytosolic, mitochondrial, and nuclear pH exclusively in the presence of β-chloro-d-alanine in living cells in a concentration-dependent manner. The pH-Control approach is promising for investigating the ultralocal pH imbalance associated with many diseases.