Development of a Chemogenetic Approach to Manipulate Intracellular pH

Ghaffari Zaki A., Miri S. M., Çimen Ş., Akgül Çağlar T., YİĞİT E. N., AYDIN M. Ş., ...More

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol.145, no.22, pp.11899-11902, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 145 Issue: 22
  • Publication Date: 2023
  • Doi Number: 10.1021/jacs.3c00703
  • Journal Name: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Compendex, Computer & Applied Sciences, DIALNET
  • Page Numbers: pp.11899-11902
  • Istanbul Medipol University Affiliated: Yes

Abstract

Chemogenetic Operation of iNTRacellular prOton Levels (pH-Control) is a novel substrate-based enzymatic method that enables precise spatiotemporal control of ultralocal acidification in cultured cell lines and primary neurons. The genetically encoded biosensor SypHer3s showed that pH-Control effectively acidifies cytosolic, mitochondrial, and nuclear pH exclusively in the presence of β-chloro-d-alanine in living cells in a concentration-dependent manner. The pH-Control approach is promising for investigating the ultralocal pH imbalance associated with many diseases.