Aptamer and nanomaterial based FRET biosensors: a review on recent advances (2014–2019)

Pehlivan Z. S., Torabfam M., KURT H., Ow-Yang C., Hildebrandt N., Yüce M.

Microchimica Acta, cilt.186, sa.8, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 186 Sayı: 8
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1007/s00604-019-3659-3
  • Dergi Adı: Microchimica Acta
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Biosensors, ssDNA, FRET, Up-converting nanoparticles, Quantum dots, Gold nanoparticles, Graphene, Graphene quantum dots, Carbon dots, Reduced graphene oxide, Metal-organic framework
  • İstanbul Medipol Üniversitesi Adresli: Evet

Özet

Fluorescence resonance energy transfer, one of the most powerful phenomena for elucidating molecular interactions, has been extensively utilized as a biosensing tool to provide accurate information at the nanoscale. Numerous aptamer- and nanomaterial-based FRET bioassays has been developed for detection of a large variety of molecules. Affinity probes are widely used in biosensors, in which aptamers have emerged as advantageous biorecognition elements, due to their chemical and structural stability. Similarly, optically active nanomaterials offer significant advantages over conventional organic dyes, such as superior photophysical properties, large surface-to-volume ratios, photostability, and longer shelf life. In this report (with 175 references), the use of aptamer-modified nanomaterials as FRET couples is reviewed: quantum dots, upconverting nanoparticles, graphene, reduced graphene oxide, gold nanoparticles, molybdenum disulfide, graphene quantum dots, carbon dots, and metal-organic frameworks. Tabulated summaries provide the reader with useful information on the current state of research in the field. [Figure not available: see fulltext.].