Beyond the lateral resolution limit by phase imaging


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Cotte Y., Toy M. F., Depeursinge C.

Journal of Biomedical Optics, cilt.16, sa.10, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 10
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1117/1.3640812
  • Dergi Adı: Journal of Biomedical Optics
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: digital holography, microscopy, phase imaging, phase vortices, point spread function, super-resolution
  • İstanbul Medipol Üniversitesi Adresli: Hayır

Özet

We present a theory to extend the classical Abbe resolution limit by introducing a spatially varying phase into the illumination beam of a phase imaging system. It allows measuring lateral and axial distance differences between point sources to a higher accuracy than intensity imaging alone. Various proposals for experimental realization are debated. Concretely, the phase of point scatterers' interference is experimentally visualized by high numerical aperture (NA = 0.93) digital holographic microscopy combined with angular scanning. Proof-ofprinciple measurements are presented by using sub-wavelength nanometric holes on an opaque metallic film. In this manner, Rayleighs classical two-point resolution condition can be rebuilt. With different illumination phases, enhanced bandpass information content is demonstrated, and its spatial resolution is theoretically shown to be potentially signal-to-noise ratio limited. © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).