IN VITRO EVALUATION OF ANTIOXIDANT AND ANTICHOLINESTERASE ACTIVITIES OF ROSA DAMASCENA MILL. ESSENTIAL OIL AND ETHANOL EXTRACTS ROSA DAMASCENA MİLL. UÇUCU YAĞI VE ETANOL EKSTRESİNİN ANTİOKSİDAN VE ANTİKOLİNESTERAZ AKTİVİTELERİNİN İN VİTRO OLARAK DEĞERLENDİRİLMESİ
Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.50, sa.2, ss.389-397, 2026 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 50 Sayı: 2
- Basım Tarihi: 2026
- Doi Numarası: 10.33483/jfpau.1691324
- Dergi Adı: Ankara Universitesi Eczacilik Fakultesi Dergisi
- Derginin Tarandığı İndeksler: Scopus, Central & Eastern European Academic Source (CEEAS), TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.389-397
- Anahtar Kelimeler: Acetylcholinesterase, Alzheimer's disease, antioxidant activity, butyrylcholinesterase, Rosa damascena Mill
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- İstanbul Medipol Üniversitesi Adresli: Evet
Özet
Objective: Reduced acetylcholine levels, which play a critical role in learning and cholinergic neurotransmission, are closely associated with Alzheimer’s disease. This study comparatively evaluated the in vitro antioxidant activity, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory effects, and neuroprotective potential of essential oil obtained from Rosa damascena Mill. grown in the Isparta-Gönen region and the ethanol extract prepared from its dried flowers. Material and Method: Antioxidant activity was determined using DPPH and ABTS radical scavenging assays, while anticholinesterase activity was assessed by the Ellman method. Results and Discussion: The essential oil exhibited strong antioxidant activity with IC₅₀ values of 0.9 μg/ml (DPPH) and 1.1 μg/ml (ABTS), whereas the ethanol extract showed IC₅₀ values of 2.6 μg/ml (DPPH) and 2.5 μg/ml (ABTS). The essential oil also demonstrated higher enzyme inhibition (AChE: 85.1%, BChE: 80.6%) compared to the ethanol extract (AChE: 74.5%, BChE: 69.9%). Overall, the essential oil showed superior antioxidant and anticholinesterase activities. These findings support the traditional use of rose oil for memory enhancement and suggest its potential as a natural source for pharmaceutical formulations targeting neurodegenerative disorders.