Modification of Polydivinylbenzene Microspheres by a Hydrobromination/Click-Chemistry Protocol and their Protein-Adsorption Properties
Macromolecular Bioscience, cilt.11, sa.1, ss.141-150, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 11 Sayı: 1
- Basım Tarihi: 2011
- Doi Numarası: 10.1002/mabi.201000280
- Dergi Adı: Macromolecular Bioscience
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.141-150
- Anahtar Kelimeler: Biomolecule immobilization, Click chemistry, Microspheres, Poly(divinylbenzene), Surface modification
- İstanbul Medipol Üniversitesi Adresli: Evet
Özet
Hydrophobic- and/or hydrophilic-polymer-grafted PDVB microspheres are synthesized by the combination of hydrobromination and click-chemistry processes. The modified-PDVB microspheres and the intermediates at various stages of synthesis are characterized using GPC, 1H NMR and FTIR spectroscopy and TGA analysis. Use of the microspheres as a support matrix for reversible protein immobilization via adsorption is investigated. The system parameters such as the adsorption conditions (i.e., enzyme concentration, medium pH) and desorption are studied and evaluated with regards to the biocatalytic activity and adsorption capacity.The synthesis of PDVB cross-linked core microspheres carrying hydrophilic and/or hydrophobic chains on the surface is reported. The microspheres are characterized and used as a support matrix for the reversible immobilization of Agaricus bisporus laccase. Adsorption conditions and desorption are investigated. The system parameters such as adsorption conditions and desorption were investigated and evaluated with regard to the biocatalytic activity and adsorption capacity. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.