Can chondral healing be improved following microfracture? The effect of adipocyte tissue derived stem cell therapy

Ceylan H. H., Bilsel K., Buyukpinarbasili N., CEYLAN H., Erdil M., Tuncay I., ...More

Knee, vol.23, no.3, pp.442-449, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 3
  • Publication Date: 2016
  • Doi Number: 10.1016/j.knee.2015.11.008
  • Journal Name: Knee
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.442-449
  • Keywords: Adipocyte tissue derived stem cell, Osteochondral defect, Microfracture, Chondral healing, Cartilage repair
  • Istanbul Medipol University Affiliated: Yes


Background: We aimed to investigate the effect of adipose tissue-derived mesenchymal stem cells (ADSCs) on chondral healing using the microfracture (MF) technique. Methods: Thirty male rabbits were randomly divided into three groups. Standard cylindrical osteochondral defects (OCDs) were created in the weight-bearing areas of the medial condyles of all the right knees; the defects were four millimeters in diameter and two millimeters in depth. The control group (group A) was restricted to spontaneous healing. For group B, we performed MF with a 1.5-mm drill. For group C, we applied MF using the same method and then applied 3 × 106 ADSCs to the defect area. At eight weeks post-operation, the subjects were sacrificed, and the distal femoral joint surfaces were evaluated histopathologically for chondral healing. The samples were scored according to the International Cartilage Repair Society (ICRS) scale. Results: The results for group C were significantly better than those for group A in terms of the surface properties (p = 0.003). The matrix evaluation was better for group A than for group C (p = 0.01). The cell distribution, cell viability and subchondral bone parameters were similar between the groups (p = 0.198, p = 0.387 and p = 0.699). The cartilage mineralization parameter was better for group C than for group A (p = 0.001). The signs of healing were better for group C than for group B, but the differences were not significant (p = 0.185). Conclusions: Improvements with additional ADSC treatments were not statistically significant in cases in which ADSC treatment was compared with isolated MF treatment. Clinical Relevance: Additional ADSCs treatment may have positive effect on chondral healing but it doesn't seem significant.