Coexistence of blaOXA-48 and aac(6′)-Ib-cr genes in Klebsiella pneumoniae isolates from Istanbul, Turkey

Nazik H., Öngen B., METE B., Aydin S., Yemişen M., Keleşoǧlu F., ...More

Journal of International Medical Research, vol.39, no.5, pp.1932-1940, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 5
  • Publication Date: 2011
  • Doi Number: 10.1177/147323001103900538
  • Journal Name: Journal of International Medical Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1932-1940
  • Keywords: QUINOLONES, CARBAPENEMS, beta-LACTAMASE, QUINOLONE RESISTANCE, CARBAPENEM RESISTANCE, KLEBSIELLA PNEUMONIAE, PLASMIDS, OXA-48
  • Istanbul Medipol University Affiliated: No

Abstract

This study evaluated the presence of carbapenem hydrolysing β-lactamase genes and plasmid-mediated quinolone resistance (PMQR) determinants in 22 Klebsiella pneumoniae isolates collected from the Istanbul Medical Faculty, Turkey, which reduced the susceptibility or resistance to carbapenem. The VITEK® 2 system and E-tests were used to determine the minimum inhibitory concentrations needed to inhibit bacterial growth. Genes were screened by polymerase chain reaction, and gene transferability was evaluated by transconjugation. Strain clonality was investigated by pulsed-field gel electrophoresis (PFGE). All strains were OXA-48 β-lactamase producers and three (13.6%) were also positive for the aac(6′)- Ib-cr gene. Most of the strains harboured other b-lactamase (bla) genes such as blaTEM′, blaSHV′aCTX-M and blaVEB-1. The transconjugants mostly harboured blaOXA-48 and other β-lactamases separately. PFGE revealed eight pulsotypes among the isolates. The coexistence of blaOXA-48 and PMQR in K. pneumoniae isolates may present a significant threat to health, especially in the nosocomial setting. © 2011 Field House Publishing LLP.