Posıtıve effect of platelet rich fibrin on osseointegration

ÖNCÜ E., Bayram B., Kantarcı A., GÜLSEVER S., Alaaddinoğlu E.

Medicina Oral, Patologia Oral y Cirugia Bucal, vol.21, no.5, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 5
  • Publication Date: 2016
  • Doi Number: 10.4317/medoral.21026
  • Journal Name: Medicina Oral, Patologia Oral y Cirugia Bucal
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Bone regeneration, Dental implants, Matrix for growth factors, Osseointegration, Platelet rich fibrin
  • Istanbul Medipol University Affiliated: Yes


Background: Leukocyte-platelet rich fibrin (L-PRF) is a second generation platelet concentrate clinically used to accelerate tissue healing and bone regeneration. Achieving reduced implant osseointegration time could provide immediate or early loading of implants. The aim of this study was to evaluate the L-PRF-induced osseointegration and bone-implant contact (BIC) in an experimental animal model. Material and Methods: Twelve 4-month-old New Zealand white rabbits were used. Following general anesthesia, 3-5 mL of blood was obtained from the central artery in rabbit ear and L-PRF was prepared. Two implant cavities (5 mm long and 3 mm in diameter) were created in each tibia with a total of four cavities in each animal. Two of these cavities were selected and covered with PRF (test group). The remaining L-PRF was used to soak the implants placed into the L-PRF covered sockets. Other cavities were left as controls. In total, 48 implants were placed. Animals were sacrificed after two, three, or four weeks. Histological samples were obtained and periimplant tissues were histomorphometrically evaluated for bone-to-implant contact and new bone formation. Results: Histomorphometric analyses of the defects revealed that the L-PRF was detectable up to the second week. Application of L-PRF increased the rate and amount of new bone formation in the experimental group compared to the control group. Bone-to-implant contact was enhanced when the surface was pre-wetted with LPRF (p<0.01). Conclusions: The results of this study demonstrated that L-PRF application may increases amount and rate of new bone formation during the early healing period and provides a faster osseointegration around implants.